Discussion on the principle and treatment pathway of 's three-needle technique for mind regulation and treatment from the "adjusting to regulate mind, adjusting blood to regulate mind" / 中国针灸

The 's three-needle technique is famous for mind regulation and treatment. From the perspective of and blood, the special principle and treatment pathway of 's three-needle technique for mind regulation and treatment were summarized and analyzed. Moreover, themethods of invigorating brain and regulating mind, calming heart and tranquilizing mind, strengthening spleen and stomach, dredging the meridian and channel were applied to guide the into the brain to regulate the mind. The mind is regulated by adjusting heart and blood, nourishing the with , adjusting the source of blood and and dredging the channel of and blood. With "adjusting to regulate mind, adjusting blood to regulate mind", a comprehensive treatment is achieved to increase the efficacy of mind regulation and treatment.

Influencing factors of the occurrence of propagated sensation effects at Zusanli (ST 36) based on artificial intelligence technology / 中国针灸

OBJECTIVE@#To explore the influencing factors of the occurrence of propagated sensation effects at Zusanli (ST 36) based on artificial intelligence technology.@*METHODS@#A total of 272 undergraduates and postgraduates of Guangzhou University of TCM were recruited. The basic information, including TCM constitution, body weight index (BMI), pulse, tongue and coating, face complexion, as well as the occurrence of propagated sensation effects at Zusanli (ST 36) with different acupuncture needles and techniques, were recorded. The repeated incremental pruning to produce error reduction (RIPPER) algorithm was applied to predict the results and summarize the rules of relevant factors.@*RESULTS@#① The propagated sensation effects were often observed in the subject with -deficiency constitution (55 cases in total, of them 1 case was misclassified). ② The propagated sensation effects were observed in the subject with balanced - constitution (39 cases in total, of them 0 case was misclassified). ③ The propagated sensation effects were observed in the subject with phlegm-damp constitution combined with thin tongue and white coating (31 cases in total, of them 8 cases were misclassified). ④ The propagated sensation effects were observed in the subject with phlegm-damp constitution combined with light tongue coating (7 cases in total, of them 2 cases were misclassified). ⑤ The propagated sensation effects were observed in all the subject with special constitution (6 cases in total, of them 0 cases were misclassified). ⑥ The propagated sensation effects were not observed in the subject with other constitution or the combination of constitution and symptoms (134 cases in total, of them 4 cases were misclassified). The accuracy rate of classification was 94.49%. The difference of acupuncture needles and techniques was not significant in propagated sensation effects (>0.05).@*CONCLUSION@#The TCM constitution may be the most essential factor in the occurrence of propagated sensation effects.

Clinical Observation of Suspending-needle Therapy Combined with Modified Zhengan Xifeng Decoction for Treatment of Primary Facial Spasm / 广州中医药大学学报

Objective To observe the therapeutic effect of suspending-needle therapy combined with modified Zhengan Xifeng Decoction for the treatment of primary facial spasm,and to explore an effective traditional Chinese medicine therapy for primary facial spasm.Methods A total of 65 qualified patients were randomly divided into trial group (33 cases) and control group(32 cases),which was treated with suspending-needle therapy combined with modified Zhengan Xifeng Decoction,Carbamazepine tablets respectively.Twenty-eight days constituted one course of treatment.After treatment for one course,therapeutic efficacy of the two groups was evaluated,and the degree of facial spasm was assessed.Results (1) After one course of treatment,the markedly-effective rate of the trial group was 84.8％,and that of the control group was 62.5％,the difference being significant(P ＜ 0.05).(2) After treatment,the degree of facial spasm in the two groups was much improved as compared with that of the control group (P ＜ 0.05 or P ＜ 0.01),and the improvement in the trial group was superior to that in the control group (P ＜ 0.01).Conclusion Suspending-needle therapy combined with modified Zhengan Xifeng Decoction has better effect on relieving facial spasm of the primary facial spasm patients than Carbamazepine tablets.

Systematic Review and Meta-analysis on Effect of Tai Chi Exercise on Cognitive Function of the Aged / 中医杂志

Objective To evaluate the effect of Tai Chi exercise on cognitive function of the aged.Methods Searched China National Knowledge Infrastructure database,Wanfang data knowledge service platform,Chongqing VIP Chinese science and technology periodical database,China biomedical literature database,PubMed,The Cochrane Library and Embase.Collected the clinical research literature in which the elder population practiced Tai Chi and cognitive function outcomes were researched.Conducted Cochrane bias risk assessment.Conducted systematic review and Meta-analysis for the effective information.Results Included 8 randomized controlled trials in total.All studies were described as random,but only two studies described the random method,with one study with blinding.The overall methodological quality was low.Meta-analysis results showed that,the cognitive function outcome evaluation in the aged with cognitive function defect,the overall cognitive function [MD =0.91,95％ CI (0.37,1.46),I2=0,P=0.001],language fluency [MD=2.17,95％CI (0.88,3.45),I2=0,P＜0.001] and delayed recall test [MD =0.7,95％ CI (0.29,1.11),I2 =0,P ＜0.001] evaluation in the Tai Chi group were significantly better than that in the control group,with no significant improvement in memory span [MD =0.16,95％ CI (-0.14,0.45),I2 =55 ％,P =0.30].In the outcome indicators evaluation of the healthy elder people,the overall cognitive function evaluation [MD =0.7,95％ CI (0.11,1.29),P =0.02] in the Tai Chi group was significantly better than the control group.In language fluency evaluation [MD =0.32,95％ CI (0.01,0.64),I2 =0 P =0.05],the difference was not statistically significant,but there is a potential advantage;in evaluation of execution of test line A (TMT-A) [MD =1.53,95％ CI (0.1,2.96),I2 =26％,P =0.04],the Tai Chi group was better than the control group;in evaluation of execution of test line B (TMT-B) [MD =1.24,95％CI (-5.20,7.67),I2 =94％,P =0.71] the difference between the Tai Chi group and the control group was not significant.Conclusion Tai Chi could effectively improve cognitive function of the healthy elder people and those with cognitive impairment.

Analysis of the Influencing Factors of Post-stroke Depression: Based on Machine Learning / 中医杂志

Objective To determine the influencing factors of post-stroke depression by machine learning.Methods Stroke patients' medical records (688 cases eligible) were extracted from record system,including age,gender,pulse manifestation,complexion,tongue quality,fur,Chinese medicine intervention,body mass index (BMI),blood pressure,blood glucose,blood triglyceride,blood total cholesterol,smoking history,drinking history,depression family history,stroke lesion site in imaging,as well as the final depression judgment.Single rule algorithm (1R) was adopted to learn.The risk factors influencing post-stroke patients' depression in extracted information were determined.Then the cases collected were divided into the training dataset (500 cases) and the test dataset (188cases).Optimal discriminant results were obtained by random forest model.Results Single rule algorithm showed that the most important influencing factor of post-stroke depression was stroke lesion site.By computer speculation,stroke lesions in the frontal and temporal lobes were most prone to post-stroke depression.Basal ganglia,brain stem,cerebellum,medulla oblongata and occipital lobe lesions were less likely to cause depression.The accurate classification rate could amount to 88.95％ (612/688 cases).Random forest model determination was made in the former 500cases in the training dataset.The total correct rate of determining depression was 98.2％.The total correct rate of determination in 188 cases of the test dataset was 99.47％.Six hundred and eighty-eight patients' data were learnt by random forest model.The total correct rate was 98.84％.The importance measure results showed that top 3 important indexes of post-stroke depression were lesion site,Chinese medicine intervention and depression family history.Conclusion Patients with lesions in the frontal & temporal lobes and depression family history were most prone to post-stroke depression.

Effect of Acupuncture plus Mirror Therapy on Lower-limb Dysfunction in Hemiplegia after Cerebral Infarction / 上海针灸杂志

Objective To observe the effect of JIN’s three needle therapy plus mirror therapy for knee joint movement on lower-limb function of patients with hemiplegia after cerebral infarction.Method Seventy-four patients with hemiplegia after cerebral infarction were randomized into a treatment group and a control group, 37 cases in each group. The treatment group was intervened by JIN’s three needle therapy plus mirror therapy for knee joint movement, while the control group was by mirror therapy for knee joint movement alone. After 4-week treatment, the Fugl-Meyer Assessment (FMA) and modified Barthel Index (MBI) were adopted for evaluating motor function of the affected limb and the activities of daily living (ADL), the Functional Ambulation Category scale (FAC) for assessing the walking ability, and the Twichell-Brunnstrom Stages of Stroke Recovery for determining the stage of post-stroke hemiplegia. Result After the intervention, the FMA and MBI scores were significantly changed in both groups (P<0.05). The changes of FMA and MBI scores in the treatment group after the treatment were significantly different from those in the control group (P<0.05). After the treatment, there were significant between-group differences in comparing the FAC and Brunnstrom grading results (P<0.05).Conclusion JIN’s three needle therapy plus mirror therapy for knee joint movement is an effective approach in treating hemiplegia after cerebral infarction, and it can improve the lower-limb motor function and the ADL.

Therapeutic Effect of He-Mu-Shu Point Combination Acupuncture for Patients with Functional Diarrhea and Its Influence of Anxiety / 广州中医药大学学报

Objective To observe the efficacy of electroacupuncture(EA) on the acupoint combination of He-(sea) points, front-mu points, and shu-points(abbrev. He-Mu-Shu) for the treatment of functional diarrhea, and to explore the effect of He-Mu-Shu point combination EA on the anxiety of patients. Methods A randomized controlled clinical trial was performed in 62 functional diarrhea patients. All of the patients were randomly divided into He-Mu-Shu group(32 cases) and medicine group(30 cases). He-Mu-Shu group was treated with EA on unilateral Quchi, Shangjuxu, Tianshu, Dachangshu point alternatively, and medicine group was treated with oral use of Loperamide hydrochloride capsules. Both groups were treated for 4 continuous weeks. Defecation frequency, stool property and anxiety scores were observed before treatment and 2, 4 weeks after treatment. Results After treatment, weekly defecation frequency, stool property and anxiety scores were improved in both groups, and the differences were statistically significant compared with those before treatment(P < 0.05). He-Mu-Shu point combination EA had better effect on improving weekly defecation frequency, stool property and anxiety scores of functional diarrhea patients than Loperamide hydrochloride capsules, and the differences were statistically significant (P < 0.05). Conclusion The clinical efficacy and anxiety-relief action of He-Mu-Shu point combination EA on functional diarrhea are superior to oral use of Loperamide hydrochloride capsules.

"Multi-central large-sample research on treatment of infantile anorexia with infantile massage and Chinese ;medicine based on thought of ""invigorating spleen and nourishing qi""" / 实用医学杂志

Objective To clinically observe and verificate the advantages of treating infantile anorexia of spleen-dysfunction in transportation with infantile massage and Chinese medicine based on the thought of “invigo-rating spleen and nourishing qi”. Methods Multi-central large-sample randomized controlled trial was carried out in Guangzhou, Shenzhen, and Foshan. Five hundreds cases of infantile anorexia of spleen-dysfunction in transportation were randomized into infantile massage group, Chinese medicine group, infantile massage combined with Chinese medicine group and Western medicine group and two sessions of treatment were required in each group. Separately, during the enrollment, after 2 sessions of treatment, infantile anorexia signs and symptoms scale , the related therapeutic effect criterion from clinical guideline of new drugs for traditional Chinese medicine, the expression level of hemoglobin and blood zinc were adopted for efficacy assessment. Results A mong 4 groups , the recovery rate and the total effective rate of infantile massage combined with Chinese medicine group were better than those of other groups (P < 0.05). The expression level of hemoglobin and blood zinc of 4 groups both increased obviously (all P < 0.01), but more improvement was found in infantile massage combined with Chinese medicine group (both P < 0.05). Conclusion The treatment of infantile anorexia with infantile massage and Chinese medicine based on the thought of invigorating spleen and nourishing qi has a significant effect.

Effects of Point Application on Celiac Mast Cell Degranulation in Mice with Allergic Rhinitis: An Experimental Study / 针灸推拿医学（英文版）

Objective:To investigate effects of different treatment on celiac mast cell degranulation in mice with allergic rhinitis induced with ovum albumin (OVA).Methods:60 female mice were randomly divided into five groups,12 mice in each,which were treated with different methods.Then,the celiac mast cells were separated and degranulation rate was calculated after stained with neutral red.Results:the rates of mast cell degranulation were (15±6)%,(53±11)%,(37±13)%,(31±15)%,and (47±14)% in normal group,OVA group,point application group,hormone group,and PBS group.There is obvious degranulation in the celiac mast cells of mice with OVA-induced allergic rhinitis.Point application and dexamethasone treatment could relieve the mast cell degranulation,whereas,PBS has no effect on the mast cell degranulation.Conclusion:the mechanism of antianaphylaxis of point application may lie on stabilizing the mast cell membrane,and inhibiting degranulation to reduce the inflammatory mediator.

Functional brain magnetic resonance imaging in healthy people receiving acupuncture at Waiguan versus Waiguan plus Yanglingquan points: a randomized controlled trial / 中西医结合学报

To observe the cerebral activating effects of needling at Waiguan (SJ5) versus SJ5 plus Yanglingquan (GB34) points in young healthy volunteers based on the hypothesis of "needling effect of combined acupuncture points relates to the brain activation".

Effect of dog-day moxibustion on changes of soluble cellular adhesion molecules in patients with bronchial asthma / 中国组织工程研究

BACKGROUND: It is thought in traditional Chinese medicine that dogday moxibustion (DDM) has good clinical effect in treating bronchial asthma. Study of its influence on soluble cellular adhesion molecules in patients with bronchial asthma can help to well understand its mechanism.OBJECTIVE: To observe the effect of dog-day on soluble cellular adhesion molecules in patients with bronchial asthmaDESIGN: Self-control, case-control observationSETTING: College of Acupuncture and Manipulation and Clinic of the Department of Acupuncture and Moxibustion of the First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine.PARTICIPANTS: Totally 28 patients with bronchial asthma receiving dog-day moxibustion in the Clinic of the Department of Acupuncture and Moxibustion, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from July to August 2004 were set as dog-day moxibustion group, and 10 concurrent health volunteers were set as normal control group.METHODS: According to the principle of "treating winter diseases in summer", the Chinese medicine which consists of such herbs as Baijiezi(Semen Sinapis Albae ), Mahuang(Herba Ephedrae ), Xixin (Herba Asari ),Gans ui (Radix Kansui ), Yanhu (Rhizoma Corydalis ) was stuck on the points such as Feishu, Dingchuan, Fengnen, Bailao, Shenshu. Point selection was at the two sides, and a lumpy salve was stuck on the point with adhesive tape. Three to six hours for adult and one to two hours for children in each sticking duration; and the operation was respectively done on the first (July20), second (July 30) and third (August 9) dog-day.MAIN OUTCOME MEASURES: Short-term effect 3 months later was observed, the levels of soluble cellular adhesion molecules and IgE were assayed before and 10 days after treatment, and comparison with normal control group was performed. The indexes of the patients before treatment were performed Pearson correlation analysis.RESULTS: Twenty-eight patients and 10 healthy persons all entered result analysis. [1] Intercellular adhesion molecule-1 level was significantly higher before treatment in dog-day moxibustion group than that after treatment in the dog-day moxibustion group and that in the normal control group [(281.39±64.58 ), (256.95±44.22), ( 194.30±39.05 ) μg/L,P ＜ 0.01].[2]Vaso-cellular adhesion molecule-1 level was significantly higher before treatment in the dog-day moxibustion group than that after treatment in the dog-day moxibustion group and that in the normal control group [(234.79±89.75), (185.80±84.41), (128.85±51.28)μg/L,P ＜ 0.01]. [3]IgE level was significantly higher before treatment than that after treatment in the dog-day moxibustion group and that in the normal control group [(482.61±123.43), (418.96±134.18), (161.53±43.64)μg/L,P＜ 0.01]. [4]Pearson correlation analysis result: Intercellular adhasion molecule-1 level is significantly positively correlated with IgE and vascular intercellular adhesion molecule-1 level (r =0.641,0.449 ,P ＜ 0.01,0.05). [5]Effect: Total effective rate was 93% (26/28)in the treatment of bronchial asthma with dogday moxibustion . The type of the symptoms, with blister or not, course of disease and condition of disease had no significant influence on effect (P＞ 0.05).CONCLUSION: Dog-day moxibustion may effect through inhibiting the expression of adhesion molecule or inhibiting leukocyte to adhere endothelial cell directly so as to reduce the infiltration of inflammatory cells.

Effects of Acupuncture on Expression of Neurologic Factors in Hippocampus of Depression Rats / 广州中医药大学学报

Objective To observe the effect of acupuncture on brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) expression in the hippocampus of depression rats,and to explore its possible anti-depression mechanism.Methods SD rats were randomized into the normal group,model group,acupuncture group and western medicine group.Rat models of depression were established by chronic unpredictable mild stress and by isolated raising.The western medicine group received gastric gavage of fluoxetine hydrochloride(in the dose of 2 mg?kg-1?d-1),and the acupuncture group received acupuncture on acupoints of Baihui(GV20),Xinshu(BL15) and Ganshu(BL17),once per day for 21 days.After treatment,the expression of BDNF and NGF in the hippocampus of rats was detected by immunohistochemical assay.Results The number of cells with BDNF and NGF positive expression in the hippocampus of model group was decreased(P

Effect of Acupuncture on Estrogen,Estrogen Receptor and Estrogen Signal Transduction in Rat Model of Hyperplasia of Mammary Glands / 广州中医药大学学报

0.05).After treatment,the nipple height was increased in the model group(P

Effect of Electroacupuncture on Learning and Memory in Vascular Dementia Rats and Its Mechanism / 广州中医药大学学报

【Objective】 To observe the effect of electroacupuncture(EA) onlearning and memoryin vascular dementia (VD) rats andto explore its possible therapeutic mechanism.【Methods】Fifty specific pathogenfree(SPF) adult rats were randomizedintofour groups.Except the 8 rats in the pseudo-operation group(group A),the other 42 rats were induced cerebral ischemia by four-vessel occlusion method.Then the model rats were randomized into model group (group B),EAgroup(group C) and nimotop group(group D).Groups Aand B were fed with the same forage and received no medication.Group Creceived EAonthe acupoints of Baihui,Geshu,Pishu and Shenshu,and EAwas set in continuous wave with the frequency being 150Hz,once per day and lasting 15 consecutive days.Group Dreceived nimotop 12mg/kg,qd,for 15 consecutive days.After 15-day treatment,the learning and memory abilities of the rats were observed by Morris water test,the plasma carbon monoxide(CO) level was detected by double-wavelength spectrophotometer,and brain nitric oxide(NO) content was examined byspectrophotometry-colorimetry.【Results】The model rats had obvious disorders of learningand memory,their escapelatency was prolonged,thetimes swimmingacross the platforminthe original quadrant did not differ fromthose inthe other three quadrants,and plasma COand cerebral NOlevels were obviouslyincreased.In EAgroup,escapelatency was shortened,thetimes swimmingacrossthe platform inthe original quadrant were more thanthose inthe other three quadrants,and plasma COand cerebral NOlevels were obviously decreased(P

Immune Mechanism of Acupuncture for Dysmenorrhea in Rats / 广州中医药大学学报

【Objective】 To observe the effect of acupuncture on immune organs(thymus and spleen) and T lymphocyte subtypes of CD3,CD4 and CD8 in rats with dysmenorrhea.【Methods】Thirty SD rats were randomized into normal control group,model group and electroacupunture(EA) group.Except the normal control group,rats in other groups received intraperitoneal injection of diethylstilbestrol and oxytocin to induce dysmenorrhea.EA group received EA on bilateral acupoints of Sanyinjiao and Guanyuan additionally.After 10-day treatment,body-stretching rate,the pathological changes of thymus and spleen,and blood CD3,CD4 and CD8 levels in the three groups were observed.【Results】In the model group,body-stretching rate was 100%,the pathological changes of thymus and spleen were obvious,and blood CD3 and CD4 levels were decreased as compared with the normal control group(P

Effect of Heat Shock Protein 70 Expression Induced by Herba Hedyotis Diffusae on Apoptosis of H22 Hepatoma Cells / 中药新药与临床药理

Objective To observe the effect of heat shock protein 70 (HSP70) expression induced by Herba Hedyotis Diffusae (HHD) on the apoptosis of H22 hepatoma cells.Methlds Mice were immunized with H22 hepatoma cells from in-vivo ascites of the mice pretreated by HHD and Radix Codonopsis (RC).Mice were divided into five groups after being blocked with HSP70 monoclonal antibody:non-HHD blocking group,HHD blocking group,non-RC blocking group,RC blocking group and the blank control group,10 mice in each groups.Then the mice were attacked with cultured H22 hepatoma cells to induce transplanted tumor.The apoptosis in the transplanted tumor was detected by a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) method.Results Large amount of brown particles can be observed in the cell nucleus of transplanted tumor mice from HHD groups.Compared with RC groups,number of apoptotic cells and the staining intensity were increased in HHD groups.The apoptosis in HHD groups and RC groups differed from that in the blank control group,and was obvious in HHD groups.The apoptosis exerted a increasing tend in non-HHD blocking group,but the difference was insignificant between non-HHD blocking group and HHD blocking group,and between non-RC blocking group and RC blocking group.Conclusion HHD can promote the apoptosis of H22 hepatoma cells in transplated tumor through inducing the expression of HSP70 to some extent,and the related mechanism of inducing apoptosis needs further research.

Influence of Electroacupuncture Therapy of Activating Du Meridian,Regulating Mind and Reinforcing the Fundamental on Learning and Memory Ability and Plasma Endothelin Level in Rats with Vascular Dementia / 广州中医药大学学报

Objective To observe the effect of electroacupuncture(EA) therapy of activating Du meridian,regulating mind and reinforcing the fundamental on learning and memory ability and plasma endothelin(ET) level in rats with vascular dementia(VD).Methods Healthy adult SD rats were randomized into pseudo-operation group(N=8),EA group(N=9,EA on the acupoints of Baihui,Dazhui,Pishu and Shenshu),nimodipine group(N=8,at the dose of 12mg/kg) and the model group(N=8).Rat models of VD were established by the modified 4-vascular occlusion(4-VO)method.Morris water maze test was used for the behavioral observation,and plasma ET level was measured by radioimmunoassay(RIA).Results Plasma ET level was increased obviously,the disorder of learning and memory ability was obvious and the escape latency in water maze was prolonged in the model group(P0.05).In EA group and nimodipine group,the escape latency was shortened(P0.05 compared with the pseudo-operation group).Conclusion The therapeutic mechanism of EA therapy of activating Du meridian,regulating mind and reinforcing the fundamental for VD is probably related with the regulation of plasma ET level and with the increase of learning and memory ability.

Effect of Acupoint Thread-embedding Therapy on Electrogastrogram and Gastrointestinal Hormones in Patients with Chronic Gastritis / 广州中医药大学学报

[ Objective ] To observe the therapeutic effect of acupoint thread-embedding therapy for chronic gastritis. [Methods] Seventy cases of chronic gastritis were randomized into group A ( n = 36, treated with acupoint thread-embedding therapy) and group B (n = 34, treated with acupuncture); the changes of electrogastrogram (ECG) and plasma contents of gastrin (Gas) and substance P (SP) were compared before and after treatment. [Results] The effective rate was 88.89% in group A and 76.47% in group B, the difference being significant ( P 0.05 ) while postprandial ECG frequency differed from that before treatment ( P 0.05). [Conclusion] Acupoint thread-embedding therapy exerts certain effect for the treatment of chronic gastritis; its therapeutic mechanism may be related to the regulation of gastrointestinal function by regulating gastrointestinal hormone levels and by increasing ECG amplitude and frequency.

Relationship Between Syndrome Patterns of Traditional Chinese Medicine and Apoptosis- Related Oncogene mRNA Expressions in Precancerous Lesions of Gastric Cancer / 广州中医药大学学报

[Objective] To study the relationship between syndrome patterns of traditional Chinese medicine and bcl- 2 oncogene, p53 suppression gene mRNA expressions in precancerous lesions of gastric cancer (PIGC). [Methods] Forty PLGC cases were endoscopically and pathologically confinned, including 24 cases of moderate dysplasia of gastric mucosa, 9 cases of severe dysplasia, 7 cases of incomplete colonic intestinal metaplasia. Of the 40 cases, 10 were complicated with Qi stagnation, 12 with stomach-heat, and 18 with blood stasis, mRNA expression of bcl- 2 oncogene and p53 suppression gene were detected in situ by molecular hybridization method. [Results] The mRNA overexpression of bcl- 2 oncogene and p53 suppression gene were found in PLGC, and the expression was gradually increased with the progress of lesions. In the complicated cases, the mRNA expression of bcl- 2 oncogene and p53 suppression gene were the least in the cases with Qi stagnation and less in the cases with stomach- heat than in the cases with blood stasis. [Conclusion] Abnormal mRNA expression of bcl- 2 oncogene and p53 suppression gene were found in PLGC cases and related with different complicated cases, indicating the specificity of different syndrome patterns.

Effect of Acupuncture Combined with Guishen Decoction on Serum ASAb Level ofRat Models with Immulogical Infertility / 广州中医药大学学报

Objective To explore the effect of acupuncture combined with Guishen Decoction (GD) on serum antisperm antibody (ASAb) level of rat models with immunological infertility. Methods Active immunization was used to establish rat models with positive serum ASAb. Eighty SD rats were alloc ated to four group s: Group A (treated with acupuncture and GD), Group B( treated with GD), Group C (treated with acupuncture), Group D (model control group). GD is composed of Ra dix Re hmanniae Praeparata, Semen Cuscutae, Fructus Ligustri Lucidi, Rhizoma Dioscorae, Fructus Lycii, Ramulus Loranthi, etc.. Results The negative rate of serum A SAb in Group A was significant higher than that in Group B and Group C (P